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Image Search Results
Journal: Inflammation and Regeneration
Article Title: Novel artificial nerve transplantation of human iPSC-derived neurite bundles enhanced nerve regeneration after peripheral nerve injury
doi: 10.1186/s41232-024-00319-4
Figure Lengend Snippet: Early-stage tissue evaluation after neurite bundle-derived artificial nerve transplantation. a , b Immunohistochemistry images with anti-NFH and anti-CD31 antibodies. Dotted arrows indicate the proximal side of the intact nerve areas, and solid arrows indicate the NFH- and CD31-positive regeneration tip in the reconstructed tissue. Scale bars = 1 mm. c , d Quantitative comparison of axonal extension distance was indicated by NFH-positive areas and that of vascular elongation distance was demonstrated by CD31-positive areas at 1 and 2 weeks after transplantation. Significant differences between groups elongation distances were observed in 2-week samples only. e , f Iba1 immunohistochemistry image of the proximal part of the regenerated tissue at 2 weeks after transplantation (e). Scale bars = 1 mm. Quantitative analysis of Iba1-positive areas demonstrated that a larger number of macrophages aggregated in the Auto, motor TP and sensory TP (f). ( n = 3). * p < 0.05, ** p < 0.01, N.S. = not significant. Data are represented as the mean ± SEM
Article Snippet: The primary antibodies used in this study were human polyclonal anti-pan-ELAVL (ELAV-like protein 2/3/4) antibody (1:2000, kindly provided from Prof. Robert Darnell, Rockefeller University), rabbit polyclonal anti-TrkA antibody (1:500, kindly provided from Prof. Louis F. Reichardt, UCSF), chicken polyclonal anti-TrkB antibody (1:500, kindly provided from Prof. Louis F. Reichardt, UCSF), goat polyclonal anti-TrkC antibody (AF373, 1:200, R&D systems), rabbit polyclonal anti-parvalbumin antibody (PV-28, 1:1000, Swant), rabbit polyclonal anti-CGRP antibody (BML-CA1134, 1:500, Enzo Life Sciences), Goat polyclonal anti-Choline acetyltransferase antibody (AB144P, 1:200, Chemicon), Mouse monoclonal anti-Islet1 and Islet2 antibody (39.4D5, 1:200, DSHB), rabbit polyclonal anti-Neurofilament heavy polypeptide antibody (ab8135, 1:500, Abcam), goat polyclonal anti-mouse and anti-rat CD31 (AF3628, 1:100, R&D),
Techniques: Derivative Assay, Transplantation Assay, Immunohistochemistry, Comparison
Journal: Leukemia
Article Title: HLXB9 activates IL6 in Hodgkin lymphoma cell lines and is regulated by PI3K signalling involving E2F3.
doi: 10.1038/sj.leu.2403716
Figure Lengend Snippet: Figure 3 Expression of IL6 and HLXB9 by RT-PCR in response to HLXB9 modulation. (a) Western blot control of expression vector pcrHLXB9 in comparison to empty vector pcDNA3 transfected in 293 cells. GDM-1 positive control cells strongly express HLXB9.29 (b) HL cell line L-540 was treated with 107 M TPA or 0.1% DMSO in combination with transfection of pcrHLXB9. Expression of IL6 is detectable after TPA treatment only. Transfection with pcrHLXB9 enhances expression of IL6. (c) HDLM-2 cells were treated with antisense-oligo (AS) directed against HLXB9 in comparison to control- oligo (AS-control). Both HLXB9 and IL6 evidenced reduced expres- sion. NTC: no template control.
Article Snippet: Inhibition of secreted IL6 protein was performed using
Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Control, Plasmid Preparation, Comparison, Transfection, Positive Control
Journal: Leukemia
Article Title: HLXB9 activates IL6 in Hodgkin lymphoma cell lines and is regulated by PI3K signalling involving E2F3.
doi: 10.1038/sj.leu.2403716
Figure Lengend Snippet: Figure 4 Schematic diagram of IL6 promoter constructs. IL6 promoter sequences were cloned before a reporter gene (EGFP). Potential binding sites for AP-1, NFkB, and GBX2/HLXB9 (EHG) are indicated.
Article Snippet: Inhibition of secreted IL6 protein was performed using
Techniques: Construct, Clone Assay, Binding Assay